Аnalyzing buckwheat genotypes obtained on selective media with zinc in vitro using ISSR markers

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The paper evaluates four ISSR-markers (М1, М2, М7, and М11) for their effectiveness in research on the genetic distances of regenerated Fagopyrum esculentum plants, which were obtained in vitro on selective media with high doses of ZnSO4×7 H2O (808–1313 mg/L). The level of polymorphism was determined to be high in the combined sample (74.4%). The obtained speciemens might be used in the breeding of new varieties with economically important traits.

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作者简介

S. Borovaya

Chaikа Federal Scientific Center of Agricultural Biotechnology of the Far East

编辑信件的主要联系方式.
Email: borovayasveta@mail.ru
俄罗斯联邦, Primorsky Krai, Timiriasevsky, 692539

A. Klykov

Chaikа Federal Scientific Center of Agricultural Biotechnology of the Far East

Email: borovayasveta@mail.ru
俄罗斯联邦, Primorsky Krai, Timiriasevsky, 692539

N. Boginskaya

Chaikа Federal Scientific Center of Agricultural Biotechnology of the Far East

Email: borovayasveta@mail.ru
俄罗斯联邦, Primorsky Krai, Timiriasevsky, 692539

参考

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  7. Aldaej M.I., Rezk A.A., El-Malky M., Shalaby T.A. Comparative genetic diversity assessment and Marker-Trait Association using two DNA marker systems in rice (Oryza sativa L.) // Agronomy. 2023. V. 13. № 2. https://doi.org/10.3390/agronomy13020329
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  10. Sabreena N. М., Mahajan R., Hashim M.J. et al. Deciphering allelic variability and population structure in buckwheat: An analogy between the efficiency of ISSR and SSR markers // Saudi J. Biol. Sci. 2021. V. 28. Р. 6050–6056. https://doi.org/10.1016/j.sjbs.2021.07.061
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2. Fig. 1. UPGMA is a dendrogram based on the values of genetic distances for buckwheat regenerants. 1 is a group of variants characterized by the smallest DN compared to the original form; 2 is a group of variants characterized by the largest DN compared to the original form.

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