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Effect of pCMV-VEGF165 plasmides on the reparation of full-layer skin wound in experiment

Genes & Cells: Vol XIV, №4, 2019, pp: 25-28 DOI: 10.23868/201912028


V.G. Shestakova, V.V. Banin, R.V. Deev, D.V. Bazhenov

The study of the regeneration of a full-layer skin wound under conditions of stimulated angiogenesis was carried out by paravulnare injection of a solution containing the plasmid pCMV-VEGF165. The inflammatory reaction in the experimental group of rats was more intense and already on day 7, the leukocyte shaft in the control was 1.3 times wider, and on day 14 in the experimental group, this indicator was 2.2 times less than the control figures. The indicators characterizing the development of granulation tissue on day 7, in the second group, 1.3 prevail over the control values, after 14 days, due to the maturation of young connective tissue — 1.1 times. The greatest differences in the comparison groups were found in relation to the degree of epithelialization of the defect. The epithelium thickness at the border is 1.4 times the control, the epithelial layer thickness is 1.7 times, and the length is 1.5 times. Compared with the control values, the number of vessels in the experimental group on day 7 were 2.3 times higher, on day 14 — 1.8 times and on day 21 — 1.7 times. The healing of the full-thickness wound of the skin in the experimental group proceeded more intensively compared with the control, and epithelization was completed 2 days earlier. In addition, stimulation of angiogenesis led to the formation of an organ-specific regenerate practically indistinguishable from intact skin.

Keywords: regeneration, stimulated angiogenesis, granulation tissue, epithelium.

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