On the resistance of the green alga Ulva Lactuca L. and associated microorganisms to the effects of diesel fuel under experimental conditions

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Abstract

It has been experimentally observed that Ulva lactuca from the Barents Sea, is capable of surviving in a laboratory environment when the concentration of water soluble fractions of diesel fuel in water is up to 1 mg/l. A concentration of more than 4.9 mg/l was critical for algae. During these treatments, decreases in photosynthetic rate (2-16 times relative to the control) and the amount of photosynthetic pigments (more than 2 times of the control), an increase the hydrogen peroxide content and the activity of superoxide dismutase were observed in macrophytes. The number, biomass and size characteristics of heterotrophic bacteria associated with algae changed significantly. Thus, the high content of petroleum products in the environment limits the spread of U. lactuca algae, however, with minor contamination, they can actively participate in bioremediation processes.

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About the authors

D. V. Pugovkin

Murmansk Marine Biological Institute of the Russian Academy of Sciences (MMBI RAS)

Author for correspondence.
Email: pugovkin2005@yandex.ru
Russian Federation, Vladimirskaya 17, Murmansk, 183010

I. V. Ryzhik

Murmansk Marine Biological Institute of the Russian Academy of Sciences (MMBI RAS); Murmansk Arctic University (MAU)

Email: pugovkin2005@yandex.ru
Russian Federation, Vladimirskaya 17, Murmansk, 183038; Sportivnaya 13, Murmansk, 183010

D. O. Salakhov

Murmansk Marine Biological Institute of the Russian Academy of Sciences (MMBI RAS)

Email: pugovkin2005@yandex.ru
Russian Federation, Vladimirskaya 17, Murmansk, 183038

M. P. Venger

Murmansk Marine Biological Institute of the Russian Academy of Sciences (MMBI RAS)

Email: pugovkin2005@yandex.ru
Russian Federation, Vladimirskaya 17, Murmansk, 183038

G. M. Voskoboynikov

Murmansk Marine Biological Institute of the Russian Academy of Sciences (MMBI RAS)

Email: pugovkin2005@yandex.ru
Russian Federation, Vladimirskaya 17, Murmansk, 183038

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Supplementary files

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2. Fig. 1. Gross content of water-soluble fractions of diesel fuel with the addition of 50 mg/l of diesel fuel in the presence of algae and without them.

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3. Fig. 2. Changes in the cellular apparatus of cells during the experiment at different concentrations of diesel fuel: a – control – 0 days; b – 6 days – 5 mg/l. Epiphytic microorganisms are noted at the periphery; c – 6 days – 100 mg/l. Many bacteria are noted at the periphery. The greater part of the section is granular content. d – 10 days – 50 mg/l. b – epiphytic bacteria, p – perinoids, s – starch granules.

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4. Fig. 3. Intensity of photosynthesis of Ulva lactuca during the experiment under the influence of different concentrations of diesel fuel.

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5. Fig. 4. Content of photosynthetic pigments after 10 days of exposure to different concentrations of DT: a – chlorophyll (A and B); b – carotenoids.

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6. Fig. 5. Activity of the antioxidant system under the influence of different DTs after 10 days of the experiment. a – hydrogen peroxide content in U. lactuca cells after 10 days of the experiment; b – SOD activity; c – catalase activity.

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7. Fig. 6. Total bacterial count (TBC), the number of cultivated heterotrophic bacteria (CHB) and the bacterial biomass of epiphytic bacteria of the alga Ulva lactuca.

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