Sequencing and annotation of the chloroplast genome of Triticum timonovum Heslot et Ferrary

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Abstract

The chloroplast genome of the synthetic octaploid Triticum timonovum Heslot et Ferrary k-43065 (France) was sequenced for the first time. Plastome sequencing was carried out on a Genolab M sequencer (GeneMind, China). The genome assembly was carried out using the NOVOwrap program. The size of the chloroplast genome of T. timonovum was 136158 bp. Meanwhile, the length of the inverted repeat region was 21552 bp, the SSC region was 12795 bp. and LSC – 80257 bp. The chloroplast genomes of T. timonovum and different T. timopheevii accessions from the GenBank database were compared. As for the chloroplast genome, T. timonovum was closer to T. timopheevii (AB976560.1), but differed from it by the presence of one insert A at position 47891.

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About the authors

А. R. Kuluev

Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Author for correspondence.
Email: kuluev@bk.ru
Russian Federation, 450054, Ufa

R. T. Matniyazov

Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: kuluev@bk.ru
Russian Federation, 450054, Ufa

B. R. Kuluev

Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: kuluev@bk.ru
Russian Federation, 450054, Ufa

L. Yu. Privalov

Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: kuluev@bk.ru
Russian Federation, 450054, Ufa

A. V. Chemeris

Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences

Email: kuluev@bk.ru
Russian Federation, 450054, Ufa

References

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2. Fig. 1. a is a visual representation in the form of a ring of the sequenced chloroplast genome of T. timonovum k-43065. The genes are displayed in different colors, the blue circle in the middle shows the GC level. IRA is the inverted repeat region A, IRB is the inverted repeat region B. Genes located outside the outer circle are transcribed clockwise, and genes located inside are transcribed counterclockwise. b is a phylogenetic tree based on the alignment of nucleotide sequences of chloroplast genomes of various T. timopheevii samples from GenBank, T. turgidum MG958546.1 and T. timonovum k-43065. The Secale cereale subsp is presented as an appearance. segetale (MZ507427.1).

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